|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27392||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7072
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBB535 was a gift from Bernd Bukau (Addgene plasmid # 27392 ; http://n2t.net/addgene:27392 ; RRID:Addgene_27392)
For your References section:Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B. BMC Biotechnol. 2007 . 7():32. 10.1186/1472-6750-7-32 PubMed 17565681