Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27558||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene Plasmid 12343 (Dr. Inder Verma)
- Backbone size w/o insert (bp) 7807
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMAP3K8 (a.k.a. AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCLXSN-Tpl2/Cot was a gift from Shao-Cong Sun (Addgene plasmid # 27558 ; http://n2t.net/addgene:27558 ; RRID:Addgene_27558)
For your References section:Deregulated activation of oncoprotein kinase Tpl2/Cot in HTLV-I-transformed T cells. Babu G, Waterfield M, Chang M, Wu X, Sun SC. J Biol Chem. 2006 May 19. 281(20):14041-7. 10.1074/jbc.M512375200 PubMed 16565081