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CAV1-mEGFP/FRT/TO
(Plasmid #27762)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27762 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5069
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    caveolin-1
  • Alt name
    CAV1
  • Alt name
    cav-1
  • Species
    Canis lupus familiaris
  • Insert Size (bp)
    1329
  • GenBank ID
    NM_001003296
  • Entrez Gene
    CAV1 (a.k.a. caveolin 1)
  • Tag / Fusion Protein
    • monomeric EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The pcDNA5/FRT/TO backbone is compatible with Flp-In recombination for generation of stable cell lines and/or tet-inducible expression. Can also be used for transient expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAV1-mEGFP/FRT/TO was a gift from Ari Helenius (Addgene plasmid # 27762 ; http://n2t.net/addgene:27762 ; RRID:Addgene_27762)
  • For your References section:

    Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation. Hayer A, Stoeber M, Ritz D, Engel S, Meyer HH, Helenius A. J Cell Biol. 2010 Nov 1. 191(3):615-29. 10.1083/jcb.201003086 PubMed 21041450