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USP08 (3N3K)
(Plasmid #28115)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 28115 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pET28a-LIC (Addgene plasmid 26094)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    PDB: 3N3K, 2GFO
  • Alt name
    ubiquitin specific protease 8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Catalytic domain only.
  • Entrez Gene
    USP8 (a.k.a. HumORF8, PITA4, SPG59, UBPY)
  • Tag / Fusion Protein
    • His tag with TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site Ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments


This construct is identical to 2GFO

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    USP08 (3N3K) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28115 ; ; RRID:Addgene_28115)