R-R334W-(Kin-7)
(Plasmid #28178)

• Depositing Lab: Bernard Schimmer
• Publication: Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Backbone

• Vector backbone: pMT-REV
• Backbone size w/o insert (bp): 7600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Prkar1a
• Species: M. musculus (mouse)
• Mutation: R334W
• Entrez Gene: PRKAR1A (a.k.a. ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, Prkar1alpha, TSE1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MT1-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: G.S. McKnight, Univ. Washington, Seattle

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expression vector encoding dominant-negative regulatory subunit of type 1 cAMP-dependent protein kinase.

pMT-REV is a zinc-inducible expression vector driven by the metallothionein promoter.