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SF-1 shRNA #1
(Plasmid #28186)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 28186 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRNAT-CMV3.1/Neo
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 6478
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nr5a1 shRNA
  • Alt name
    Nr5a1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nr5a1 (a.k.a. Ad4BP, ELP, ELP-3, Ftz-F1, Ftzf1, SF-1, SF1, STF-1)
  • Tag / Fusion Protein
    • cGFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

shRNA #1 sequence: GATCCTAATGCTTGTTGTTCTGGACTTTGATATCCGAGTCCAGAACAACAAGCATTACGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SF-1 shRNA #1 was a gift from Bernard Schimmer (Addgene plasmid # 28186 ; http://n2t.net/addgene:28186 ; RRID:Addgene_28186)
  • For your References section:

    Contributions of specificity protein-1 and steroidogenic factor 1 to Adcy4 expression in Y1 mouse adrenal cells. Rui X, Tsao J, Scheys JO, Hammer GD, Schimmer BP. Endocrinology. 2008 Jul . 149(7):3668-78. 10.1210/en.2008-0203 PubMed 18388192