|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28214||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameUnc5b mouse monoclonal antibody
SpeciesD. rerio (zebrafish)
Entrez Geneunc5b (a.k.a. unc5h2)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
Terms and Licenses
See map and Genbank file for plasmid details.
Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SI1-Unc5b was a gift from Gavin Wright (Addgene plasmid # 28214 ; http://n2t.net/addgene:28214 ; RRID:Addgene_28214)
For your References section:A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357