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(Plasmid #28214)


Item Catalog # Description Quantity Price (USD)
Plasmid 28214 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 5925
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Unc5b mouse monoclonal antibody
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    unc5b (a.k.a. unc5h2)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map and Genbank file for plasmid details.

Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SI1-Unc5b was a gift from Gavin Wright (Addgene plasmid # 28214 ; ; RRID:Addgene_28214)
  • For your References section:

    A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357