|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28216||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameJamc.2 mouse monoclonal antibody
SpeciesD. rerio (zebrafish)
Entrez Genejam3a (a.k.a. jamc.2, si:ch211-277g22.3)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
See map and Genbank file for plasmid details.
Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3370-3371 and 3380-3382 when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SI4-Jamc.2 was a gift from Gavin Wright (Addgene plasmid # 28216 ; http://n2t.net/addgene:28216 ; RRID:Addgene_28216)
For your References section:A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357