|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28217||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameFlrt3 mouse monoclonal antibody
SpeciesD. rerio (zebrafish)
Entrez Geneflrt3 (a.k.a. wu:fb75d02, wu:fb93g11, wu:fc10a05, si:dkeyp-24h12.1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13_puc_Rev (Common Sequencing Primers)
See map and Genbank file for plasmid details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SI3-Flrt3 was a gift from Gavin Wright (Addgene plasmid # 28217 ; http://n2t.net/addgene:28217 ; RRID:Addgene_28217)
For your References section:A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357