pLKO human shRNA 2 c-MAF
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29433||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable from Addgene
- Backbone size w/o insert (bp) 7032
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Growth instructionsXL10 Gold Ultracompetent Cells from Stratagene or Stbl3. Grow in SOC media at 37oC
Gene/Insert namec-MAF shRNA 2
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneMAF (a.k.a. AYGRP, CCA4, CTRCT21, c-MAF)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Human c-MAF_2 shRNA: TRCN0000000257; NM_005360.x-1067s1c1
shRNA oligo sequence is - 5'-CCGGACCTGGAAGACTACTACTGGACTCGAGTCCAGTAGTAGTCTTCCAGGTTTTTT-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO human shRNA 2 c-MAF was a gift from David Sabatini (Addgene plasmid # 29433 ; http://n2t.net/addgene:29433 ; RRID:Addgene_29433)
For your References section:DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, Gray NS, Sabatini DM. Cell. 2009 May 13. ():. 10.1016/j.cell.2009.03.046 PubMed 19446321