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11.5kb-GFP (pBSK)
(Plasmid #29459)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29459 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHIP-1 promoter
  • Alt name
    SHIP1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    11500
  • Entrez Gene
    Inpp5d (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, p150Ship, s-SHIP)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See attached map ("View Map") and Materials and Methods for complete cloning details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    11.5kb-GFP (pBSK) was a gift from Larry Rohrschneider (Addgene plasmid # 29459 ; http://n2t.net/addgene:29459 ; RRID:Addgene_29459)
  • For your References section:

    The intron 5/6 promoter region of the ship1 gene regulates expression in stem/progenitor cells of the mouse embryo. Rohrschneider LR, Custodio JM, Anderson TA, Miller CP, Gu H. Dev Biol. 2005 Jul 15. 283(2):503-21. 10.1016/j.ydbio.2005.04.032 PubMed 15978570