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pET23b-proMMP-7
(Plasmid #29524)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29524 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 3700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    proMMP-7
  • Alt name
    Promatrilysin
  • Species
    H. sapiens (human)
  • Entrez Gene
    MMP7 (a.k.a. MMP-7, MPSL1, PUMP-1)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

MMP7 sequence in the plasmid is suitable for expression in bacteria but NOT in mammalian cells, as the signal sequence is not included.

The MMP7 amino acid sequence starts at amino acid 18 when compared to GenBank accession number NP_002414.1. Note that a superfluous methionine corresponding to the start signal for translation is present at the N-terminus of the expressed protein

Please note that the His tag present in the vector is not in-frame with MMP-7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23b-proMMP-7 was a gift from William Parks (Addgene plasmid # 29524 ; http://n2t.net/addgene:29524 ; RRID:Addgene_29524)
  • For your References section:

    Control of promatrilysin (MMP7) activation and substrate-specific activity by sulfated glycosaminoglycans. Ra HJ, Harju-Baker S, Zhang F, Linhardt RJ, Wilson CL, Parks WC. J Biol Chem. 2009 Oct 9. 284(41):27924-32. 10.1074/jbc.M109.035147 PubMed 19654318