Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||29524||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 3700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMMP7 (a.k.a. MMP-7, MPSL1, PUMP-1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
MMP7 sequence in the plasmid is suitable for expression in bacteria but NOT in mammalian cells, as the signal sequence is not included.
The MMP7 amino acid sequence starts at amino acid 18 when compared to GenBank accession number NP_002414.1. Note that a superfluous methionine corresponding to the start signal for translation is present at the N-terminus of the expressed protein
Please note that the His tag present in the vector is not in-frame with MMP-7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET23b-proMMP-7 was a gift from William Parks (Addgene plasmid # 29524 ; http://n2t.net/addgene:29524 ; RRID:Addgene_29524)
For your References section:Control of promatrilysin (MMP7) activation and substrate-specific activity by sulfated glycosaminoglycans. Ra HJ, Harju-Baker S, Zhang F, Linhardt RJ, Wilson CL, Parks WC. J Biol Chem. 2009 Oct 9. 284(41):27924-32. 10.1074/jbc.M109.035147 PubMed 19654318