SEMA4D (chick) pBLUE
(Plasmid
#29568)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29568 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBLUESCRIPT (SK)
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Backbone manufacturerstratagene
- Backbone size w/o insert (bp) 2900
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Vector typepBlue for ISH
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSEMA4D
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Alt nameSemaphorin 4D
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Alt nameCollapsin 4
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SpeciesG. gallus (chicken)
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Insert Size (bp)3500
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MutationFor ISH Sense: Not1 or Xho1/T3 Antisense: Kpn1 or EcoR1/T7
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GenBank IDNC_006127
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Entrez GeneSEMA4D (a.k.a. COLL-4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SEMA4D (chick) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29568 ; http://n2t.net/addgene:29568 ; RRID:Addgene_29568)