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Purpose(Empty Backbone)
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepDONR201
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Backbone manufacturerInvitrogen
- Backbone size (bp) 4470
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Vector typeGateway donor
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Unknown
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Growth instructionsDB3.1 (ccdB resistant)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Alt nameDelsGate
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GenBank IDEU360889
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains a selectable marker for U. maydis transformation and was constructed by inserting a ~2.3 kb EcoICRI carboxin
resistance (cbxR) conferring fragment from pGR3 (kindly provided by J. Hargreaves) into the blunt ended NspI site of pDONR201.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONRCBX was a gift from David_L Andrews & Scott Gold (Addgene plasmid # 29634 ; http://n2t.net/addgene:29634 ; RRID:Addgene_29634) -
For your References section:
DelsGate, a robust and rapid gene deletion construction method. Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE. Fungal Genet Biol. 2008 Apr . 45(4):379-88. 10.1016/j.fgb.2007.11.001 PubMed 18248826
