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pET-3c-rhVTN-NC
(Plasmid #30226)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 30226 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-3c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4607
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a in LB Media
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    rhVitronectin with N and C terminus cleavages
  • Alt name
    VTN
  • Alt name
    VTN-NC
  • Alt name
    rhVTN-NC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1014
  • Mutation
    deleted amino acids: 399-478, 20-62
  • Entrez Gene
    VTN (a.k.a. V75, VN, VNT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NdeI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-3c-rhVTN-NC was a gift from James Thomson (Addgene plasmid # 30226 ; http://n2t.net/addgene:30226 ; RRID:Addgene_30226)
  • For your References section:

    Chemically defined conditions for human iPSC derivation and culture. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods. 2011 Apr 10. ():. 10.1038/nmeth.1593 PubMed 21478862