|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30226||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4607
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsDH5a in LB Media
Copy numberLow Copy
Gene/Insert namerhVitronectin with N and C terminus cleavages
SpeciesH. sapiens (human)
Insert Size (bp)1014
Mutationdeleted amino acids: 399-478, 20-62
Entrez GeneVTN (a.k.a. V75, VN, VNT)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. None of the four constructs from this paper have amino acids 1-19.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-3c-rhVTN-NC was a gift from James Thomson (Addgene plasmid # 30226 ; http://n2t.net/addgene:30226 ; RRID:Addgene_30226)
For your References section:Chemically defined conditions for human iPSC derivation and culture. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Nat Methods. 2011 Apr 10. ():. 10.1038/nmeth.1593 PubMed 21478862