Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30521||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression ; Flp/FRT
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameFLP recombinase
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2500
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
driven by Xenopus laevis heat shock 70 (hsp 70) promoter
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSHSP:FLP was a gift from Gerhart Ryffel (Addgene plasmid # 30521 ; http://n2t.net/addgene:30521 ; RRID:Addgene_30521)
For your References section:Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, Senkel S, Ryffel GU, Waldner C. Transgenic Res. 2009 Aug . 18(4):595-605. 10.1007/s11248-009-9253-4 PubMed 19266305
Map uploaded by the depositor.