|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31131||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameFLP-ER ligand binding domain
SpeciesH. sapiens (human), S. cerevisiae (budding yeast)
Insert Size (bp)2300
Mutationfull length FLP linked to ligand binding domain of estrogen receptor starting at AA 251. Contains V400G and G521R.
Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
tamoxifene regulated FLP activity
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS_FLPER(T) was a gift from Gerhart Ryffel (Addgene plasmid # 31131 ; http://n2t.net/addgene:31131 ; RRID:Addgene_31131)
For your References section:FLP and Cre recombinase function in Xenopus embryos. Werdien D, Peiler G, Ryffel GU. Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3. 10.1093/nar/29.11.e53 PubMed 11376165