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Addgene

pJT122
(Plasmid #31396)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31396 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pProTet.E333
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 1682
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    pJT122
  • Alt name
    cca cluster with lacZ output plus cph8
  • Species
    Synechocystis
  • Insert Size (bp)
    9410

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AaTII (not destroyed)
  • 3′ cloning site Xba-I (not destroyed)
  • 5′ sequencing primer sequence
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJT122 was a gift from Christopher Voigt (Addgene plasmid # 31396 ; http://n2t.net/addgene:31396 ; RRID:Addgene_31396)
  • For your References section:

    Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol. 2011 Jan 14. 405(2):315-24. 10.1016/j.jmb.2010.10.038 PubMed 21035461