|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31443||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3500
Vector typeMammalian Expression ; phiC31/attB
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3000
Entrez GeneHNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
/ Fusion Protein
- FKBP L106P (N terminal on insert)
- Cloning method Restriction Enzyme
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Integration vector with attB site for phiC31 mediated integration of HNF4A2 into attP site using puromycin selection. HNF4A2 linked to destabilization domain (FKBP L106P) allowing shield inducibility.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pINT-PuroDDHNF4a2 was a gift from Gerhart Ryffel (Addgene plasmid # 31443 ; http://n2t.net/addgene:31443 ; RRID:Addgene_31443)
For your References section:Double conditional human embryonic kidney cell line based on FLP and PhiC31 mediated transgene integration. Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU. BMC Res Notes. 2011 Oct 18;4:420. 10.1186/1756-0500-4-420 PubMed 22008483