pINT-PuroDDHNF4a2
(Plasmid #31443)

• Depositing Lab: Gerhart Ryffel
• Publication: Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Backbone

• Vector backbone: pCSGFP
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression ; phiC31/attB
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HNF4A
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: HNF4A (a.k.a. FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14)
• Tag / Fusion Protein:
  • FKBP L106P (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Integration vector with attB site for phiC31 mediated integration of HNF4A2 into attP site using puromycin selection. HNF4A2 linked to destabilization domain (FKBP L106P) allowing shield inducibility.