Skip to main content
Addgene

FUW-M2-PPP1R12C S452A
(Plasmid #31679)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    FUW
  • Backbone size w/o insert (bp) 9200
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein Phosphatase 1 Regulatory Subunit 12C S452A
  • Alt name
    M2-PPP1R12C S452A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2350
  • Mutation
    changed Serine 452 to Alanine
  • Entrez Gene
    PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)
  • Tag / Fusion Protein
    • M2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer FUW F
  • 3′ sequencing primer FUW R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW-M2-PPP1R12C S452A was a gift from Anne Brunet (Addgene plasmid # 31679 ; http://n2t.net/addgene:31679 ; RRID:Addgene_31679)

  • For your References section:

    Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network of Proteins Involved in Mitosis. Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat KM, Brunet A. Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1. 10.1016/j.molcel.2011.11.005 PubMed 22137581
Related items:
Commonly requested with: