Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31714||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4716
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneADAM17 (a.k.a. ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE)
/ Fusion Protein
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5M-TACE was a gift from Rik Derynck (Addgene plasmid # 31714 ; http://n2t.net/addgene:31714 ; RRID:Addgene_31714)
For your References section:TACE-mediated ectodomain shedding of the type I TGF-beta receptor downregulates TGF-beta signaling. Liu C, Xu P, Lamouille S, Xu J, Derynck R. Mol Cell. 2009 Jul 10. 35(1):26-36. 10.1016/j.molcel.2009.06.018 PubMed 19595713