pcDNA3.1-hFrataxin-HA
(Plasmid #31895)

• Depositing Lab: Gino Cortopassi
• Publication: Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41. Epub 2007 Mar 1.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Frataxin
• Species: H. sapiens (human)
• Entrez Gene: FXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: none (unknown if destroyed)
• 3′ cloning site: none (unknown if destroyed)
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry