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CbSBS-Flag/TRRAP(S722F)
(Plasmid #32105)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32105 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32105-D50 50 μg of DNA in Tris buffer $495
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DNA 32105-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    CbS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Better growth in JM109 (Primeplus cells)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Transformation/transcription domain-associated protein (S722F)
  • Alt name
    TRRAP (S722F)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    16828
  • Mutation
    Changes serine 722 to Phe
  • Entrez Gene
    TRRAP (a.k.a. DEDDFA, DFNA75, PAF350/400, PAF400, STAF40, TR-AP, Tra1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag 1x (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site not1 (not destroyed)
  • 3′ cloning site cla1 (not destroyed)
  • 5′ sequencing primer CAG ATC GCC TGG AGA CGC C
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mutation was cloned by Vijay Walia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CbSBS-Flag/TRRAP(S722F) was a gift from Yardena Samuels (Addgene plasmid # 32105 ; http://n2t.net/addgene:32105 ; RRID:Addgene_32105)
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