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1245 pCMVb p300
(Plasmid #10717)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 10717 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    CMVbeta
  • Backbone manufacturer
    not the same as the commercial CMVb
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p300
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8000
  • GenBank ID
    U01877
  • Entrez Gene
    EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1245 pCMVb p300 was a gift from William Sellers (Addgene plasmid # 10717 ; http://n2t.net/addgene:10717 ; RRID:Addgene_10717)