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• pCol-TGM
• Sequences

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Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

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  Sequence > pCol-TGM sequence 6120 bps cccccgggctgcagatctgtagggcgcagtagtccagggtttccttgatgatgtcatacttatcctgtcc cttttttttccacagctcgcggttgaggacaaactcttcgcggtctttccagtggggatcgacggtatcg ataagcttgcagatctgcgactctagaggatctgcgactctagaggatcataatcagccataccacattt gtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaa ttgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcac aaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtc tggatctgcgactctagaggatcataatcagccataccacatttgtagaggttttacttgctttaaaaaa cctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgca gcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcatt ctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatcgagtttaccactccctatca gtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagttt accactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaag tgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagtttaccactccctatca gtgatagagaaaagtgaaagtcgagtttaccactccctatcagtgatagagaaaagtgaaagtcgagctc ggtacccgggtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtca gatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgc ggccccgaattgaccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcg agctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacgg caagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccacc ctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccg ccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgc cgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac ggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccga ccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacc cagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccg ccgggatcactctcggcatggacgagctgtacaagtaatgaaccggtcgactagggataacagggtaatt gtttgaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggattact tcttcaggttaacccaacagaaggctcgagcaaccagaattcaaggggctactttaggagcaattatctt gtttactaaaactgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaa ttaaatcacttttttcaattcacgcgttgagaacttcagggtgagtttggggacccttgattgttctttc tttttcgctattgtaaaattcatgttatatggagggggcaaagttttcagggtgttgtttagaatgggaa gatgtcccttgtatcaccatggaccctcatgataattttgtttctttcactttctactctgttgacaacc attgtctcctcttattttcttttcattttctgtaactttttcgttaaactttagcttgcatttgtaacga atttttaaattcacttttgtttatttgtcagattgtaagtactttctctaatcacttttttttcaaggca atcagggtatattatattgtacttcagcacagttttagagaacaattgttataattaaatgataaggtag aatatttctgcatataaattctggctggcgtggaaatattcttattggtagaaacaactacaccctggtc atcatcctgcctttctctttatggttacaatgatatacactgtttgagatgaggataaaatactctgagt ccaaaccgggcccctctgctaaccatgttcatgccttcttctctttcctacagctcctgggcaacgtgct ggttgttgtgctgtctcatcattttggcaaaggattcactcctcaggtgcaggctgcctatcagaaggtg gtggctggtgtggccaatgccctggctcacaaataccactgagatcgttttccctctgccaaaaattatg gggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagt gtgttggaatttctcgatcgctagcatcctaggcgcgccacatgtgagcaaaaggccagcaaaaggccag gaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagct ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggc tgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacc cggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtagg cggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgc gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctg gtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagatta tcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatg agtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcg ttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccg gaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggga agctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtg tcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccc ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagt gttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttct gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccgg cgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttc ggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaac tgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaa aaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcat ttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggtt ccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaa tttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaa tagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactcca acgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttt tttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacgg ggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaa gtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtccca ttcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctg gcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgta aaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattggagctccaccgcggtggcgg ccgctctagaactagtggatcccccgggctgcaggaattaattcgtcgacctcgaaattctaccgggtag gggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacac aagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggc cccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgca ggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagcaatggaa gcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctggga aggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggag gcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcct ttcgacctgcatccatctagatctcgatcgagcagctgaagcttatggagaagttactattccgaagttc ctattctctagaaagtataggaacttcgat

Addgene-Verified Partial Sequences (3)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

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  Sequence > EGFP-N 5'-CGTCGCCGTCCAGCTCGACCAG-3' GGTCAATTCGGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGG CGTCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGGCCTCCCACCGTACACGCCTAC CTCGACCCGGGTACCGAGCTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTC ACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGG TAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCA CTGATAGGGAGTGGTAAACTCGACTTTCACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGACTTTC ACTTTTCTCTATCACTGATAGGGAGTGGTAAACTCGATCCAGACATGATAAGATACATTGATGAGTTTGG ACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGG GGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCTCT AGAGTCGCAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACA AGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
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  Sequence > mPGK-F 5'-cattctgcacgcttcaaaag-3' TCCGGGCCTTTCGACCTGCATCCATCTAGATCTCGATCGAGCAGCTGAAGCTTATGGAGAAGTTACTATT CCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGATCCCCCTCGATCCCCCGGGCTGCAGATCTGTA GGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCG GTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGCAGATCTGCGAC TCTAGAGGATCTGCGACTCTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTA AAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTA TTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACT GCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTGCGACTCTAGAGGA TCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAA CCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAA AGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAAC TCATCAATGTATCTTATCATGTCTGGATCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGT CGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAG AGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATA
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  Sequence > EGFP-C 5'-CATGGTCCTGCTGGAGTTCGTG-3' CGGCAGGACGAGCTGTCAGTAATGAACCGGTCGACTAGGGATAACAGGGTAATTGTTTGAATGAGGCTTC AGTACTTTACAGAATCGTTGCCTGCACATCTTGGAAACACTTGCTGGGATTACTTCTTCAGGTTAACCCA ACAGAAGGCTCGAGCAAGCAGTCAGAATTCAAGGGGCTACTTTAGGAGCAATTATCTTGTTTACTAAAAC TGAATACCTTGCTATCTCTTTGATACATTTTTACAAAGCTGAATTAAAATGGTATAAATTAAATCACTTT TTTCAATTCACGCGTTGAGAACTTCAGGGTGAGTTTGGGGACCCTTGATTGTTCTTTCTTTTTCGCTATT GTAAAATTCATGTTATATGGAGGGGGCAAAGTTTTCAGGGTGTTGTTTAGAATGGGAAGATGTCCCTTGT ATCACCATGGACCCTCATGATAATTTTGTTTCTTTCACTTTCTACTCTGTTGACAACCATTGTCTCCTCT TATTTTCTTTTCATTTTCTGTAACTTTTTCGTTAAACTTTAGCTTGCATTTGTAACGAATTTTTAAATTC ACTTTTGTTTATTTGTCAGATTGTAAGTACTTTCTCTAATCACTTTTTTTTCAAGGCAATCAGGGTATAT TATATTGTACTTCAGCACAGTTTTAGAGAACAATTGTTATAATTAAATGATAAGGTAGAATATTTCTGCA TATAAATTCTGGCTGGCGTGGAAATATTCTTATTGGTAGAAACAACTACACCCTGGTCATCATCCTGCCT TTCTCTTTATGGTTACAATGATATACACTGTTTGAGATGAGGATAAAATACTCTGAGTCCAAACCGGGCC CCTCTGCTAACCATGTTCATGCCTTCTTCTCTTTCCTACAGCTCCTGGGCAACGTGCTGGTTGTTGTGCT GTCTCACTTGTT