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pcDNA6-rbNHERF1
(Plasmid #32942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32942 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32942-D50 50 μg of DNA in Tris buffer $495
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DNA 32942-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA6/HisC
  • Backbone manufacturer
    Invitrogene
  • Backbone size w/o insert (bp) 5200
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC9A3R1
  • Alt name
    NHERF
  • Species
    rabbit
  • Insert Size (bp)
    1900
  • GenBank ID
    u19815
  • Entrez Gene
    SLC9A3R1
  • Promoter CMV
  • Tags / Fusion Proteins
    • his (N terminal on backbone)
    • T7 tag (N terminal on insert)
    • partial FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer t7 foward
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NHERF-1 was cut from peGFP-rbNHERF-1 (ID#31636) constructs.
Part of the peGFP vector is present at the 3' end.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6-rbNHERF1 was a gift from Edward Weinman (Addgene plasmid # 32942 ; http://n2t.net/addgene:32942 ; RRID:Addgene_32942)

  • For your References section:

    PTH transiently increases the percent mobile fraction of Npt2a in OK cells as determined by FRAP. Weinman EJ, Steplock D, Cha B, Kovbasnjuk O, Frost NA, Cunningham R, Shenolikar S, Blanpied TA, Donowitz M. Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 30. 10.1152/ajprenal.90657.2008 PubMed 19794105
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