Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pAC-luc-TimRA
(Plasmid #33133)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33133 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAc5.1/V5-His B
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    pAc-Luciferase was constructed by ligation of the PCR product containing the Renilla Luciferase gene and an artificial poly-linker sequence into the parent vector.
  • Vector type
    Insect Expression, Luciferase
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tim-RA 3' UTR
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    tim (a.k.a. Dmel_CG3234, CG3234, Dmel\CG3234, Ritsu, TIM, Tim, Tim-1, dTIM, dTim, dtim, mel_tim, rit, s-tim, tim1)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • Firefly Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer AC5
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pAc-Luc-TimRA was constructed by amplifying the genomic region containing the clk 3′ UTR plus 300 bases downstream from the annotated end of the mRNA and ligating the PCR product into pAc-Luciferase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-luc-TimRA was a gift from Michael Rosbash (Addgene plasmid # 33133 ; http://n2t.net/addgene:33133 ; RRID:Addgene_33133)
  • For your References section:

    A role for microRNAs in the Drosophila circadian clock. Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P, Vagin VV, Zamore PD, Nelson SB, Rosbash M. Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. 10.1101/gad.1819509 PubMed 19696147