Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

L57V
(Plasmid #34556)

Full plasmid sequence is not available for this item.

Loading...

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34556 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTriEx-3
  • Backbone manufacturer
    PMID: 12864922
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    L57
  • Alt name
    LOV2-Caspase-7 fusion
  • Alt name
    Caspase-7
  • Alt name
    Photoactivated Caspase-7
  • Species
    H. sapiens (human); A.Sativa (LOV2)
  • Insert Size (bp)
    1200
  • Mutation
    catalytic domain of caspase-7 was truncated at amino acid 57
  • Entrez Gene
    CASP7 (a.k.a. CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
  • Promoter CMV, T7
  • Tag / Fusion Protein
    • YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F, T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and the monomeric YFP Venus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L57V was a gift from Kevin Truong (Addgene plasmid # 34556 ; http://n2t.net/addgene:34556 ; RRID:Addgene_34556)