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(Plasmid #34557)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 34557 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    PMID: 12864922
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    LOV2-Caspase-7 fusion
  • Alt name
  • Alt name
    Photoactivated Caspase-7
  • Species
    H. sapiens (human); A.Sativa (LOV2)
  • Insert Size (bp)
  • Mutation
    catalytic domain of caspase-7 was truncated at amino acid 57
  • Entrez Gene
    CASP7 (a.k.a. CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3)
  • Promoter CMV, T7
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer CMV-F, T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tandem fusion of LOV2 domain from A. Sativa phototropin 1, the catalytic domain of caspase-7 (human), and a mRFP variant that contains T140A and M141S mutations (introduced to facilitate cloning).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L57R was a gift from Kevin Truong (Addgene plasmid # 34557 ; ; RRID:Addgene_34557)