Stim1-CaRQ
(Plasmid #34560)

• Depositing Lab: Kevin Truong
• Publication: Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Backbone

• Vector backbone: pTriEx-3
• Backbone manufacturer: PMID: 12864922
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Stim1-CaRQ
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Q63L in RhoA
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F, T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Stim1 was cloned from Addgene plasmid 19754

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MLCK peptide - KRRWKKNFIAVSAANRFKKI
IQ peptide - GSAITVQRYVRGIQARAYARFL