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Addgene

SW Mb 64Y68F
(Plasmid #34569)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34569 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SW Mb 64Y68F
  • Alt name
    Heme reagent
  • Alt name
    WT - pMB413A
  • Species
    recombinant sperm whale
  • Insert Size (bp)
    508
  • Mutation
    His 64 to Tyr and Val 68 to Phe

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    see Addgene 20058

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expression strain: BL21 DE3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SW Mb 64Y68F was a gift from John Olson (Addgene plasmid # 34569 ; http://n2t.net/addgene:34569 ; RRID:Addgene_34569)
  • For your References section:

    His64(E7)-->Tyr apomyoglobin as a reagent for measuring rates of hemin dissociation. Hargrove MS, Singleton EW, Quillin ML, Ortiz LA, Phillips GN Jr, Olson JS, Mathews AJ. J Biol Chem. 1994 Feb 11;269(6):4207-14. PubMed 8307983
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