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pGNS-BAC
(Plasmid #34679)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34679 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pJW544
  • Backbone manufacturer
    16500
  • Backbone size (bp) 11900
  • Modifications to backbone
    Introduction of mob cassette for conjugal transfer, elimination of a BamHI site within the PBAD:trfA gene cassette, and a gentamicin resistance cassette
  • Vector type
    Bacterial Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Arabinose induction of copy number
  • Copy number
    Low Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gentamicin resistance from plasmid pBSL141 (Alexeyev, M. F., Shokolenko, I. N., Croughan, T. P., 1995. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector construction and in vitro deletion/insertion mutagenesis. Gene 160(1), 63-67.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also carries gentamicin resistance cassette. Selection for chloramphenicol and/or gentamicin resistance will maintain the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGNS-BAC was a gift from Mark Liles (Addgene plasmid # 34679)

  • For your References section:

    Gram negative shuttle BAC vector for heterologous expression of metagenomic libraries. Kakirde KS, Wild J, Godiska R, Mead DA, Wiggins AG, Goodman RM, Szybalski W, Liles MR. Gene. 2011 Apr 15;475(2):57-62. Epub 2010 Nov 26. 10.1016/j.gene.2010.11.004 PubMed 21112378
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