Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34697||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)444
MutationResidues 1-145 of human fibrillarin with K2E and A145V substitutions preceded by a linker region of SDLVPRGS. This region contains 15 arginine residues in a glycine-rich region.
Entrez GeneFBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site None (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer pGEX 3' (Common Sequencing Primers)
Terms and Licenses
The Addgene quality control sequence only verified 145 amino acids. The depositor notes that this should not be a problem for scientists using this construct as a methyl-acceptor.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-GAR was a gift from Steven Clarke (Addgene plasmid # 34697 ; http://n2t.net/addgene:34697 ; RRID:Addgene_34697)
For your References section:PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. Frankel A, Clarke S. J Biol Chem. 2000 Oct 20;275(42):32974-82. 10.1074/jbc.M006445200 PubMed 10931850