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GST-GAR
(Plasmid #34697)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 34697 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fibrillarin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Mutation
    Residues 1-145 of human fibrillarin with K2E and A145V substitutions preceded by a linker region of SDLVPRGS. This region contains 15 arginine residues in a glycine-rich region.
  • Entrez Gene
    FBL (a.k.a. FIB, FLRN, Nop1, RNU3IP1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The Addgene quality control sequence only verified 145 amino acids. The depositor notes that this should not be a problem for scientists using this construct as a methyl-acceptor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-GAR was a gift from Steven Clarke (Addgene plasmid # 34697 ; http://n2t.net/addgene:34697 ; RRID:Addgene_34697)
  • For your References section:

    PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. Frankel A, Clarke S. J Biol Chem. 2000 Oct 20;275(42):32974-82. 10.1074/jbc.M006445200 PubMed 10931850