Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBAD-PSmOrange2
(Plasmid #34957)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34957 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3963
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PSmOrange2
  • Insert Size (bp)
    747
  • Mutation
    R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
  • GenBank ID
    JQ392581 NM_001018942

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5'-atgccatagcatttttatcc
  • 3′ sequencing primer 5' GAT TTA ATC TGT ATC AGG CTG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-PSmOrange2 was a gift from Vladislav Verkhusha (Addgene plasmid # 34957 ; http://n2t.net/addgene:34957 ; RRID:Addgene_34957)
  • For your References section:

    A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. J Am Chem Soc. 2012 Aug 17. 10.1021/ja3034137 PubMed 22900938