|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34960||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Insert Size (bp)711
MutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
GenBank IDJQ392581 NM_001018942
/ Fusion Protein
- H2B (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer 5'-cggtaggcgtgtacggtgggag
- 3′ sequencing primer 5'-gttcagggggaggtgtgggagg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
https://www.ncbi.nlm.nih.gov/nuccore/JQ392581 There are D26G and V119I amino acid changes in Addgene's quality control sequence for H2B. It does not alter the function of this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pH2B-PSmOrange2 was a gift from Vladislav Verkhusha (Addgene plasmid # 34960 ; http://n2t.net/addgene:34960 ; RRID:Addgene_34960)
For your References section:A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. J Am Chem Soc. 2012 Aug 17. 10.1021/ja3034137 PubMed 22900938