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pH2B-PSmOrange2
(Plasmid #34960)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 34960 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pN1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSmOrange2
  • Insert Size (bp)
    711
  • Mutation
    R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
  • GenBank ID
    JQ392581 NM_001018942
  • Tag / Fusion Protein
    • H2B (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-cggtaggcgtgtacggtgggag
  • 3′ sequencing primer 5'-gttcagggggaggtgtgggagg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

https://www.ncbi.nlm.nih.gov/nuccore/JQ392581 There are D26G and V119I amino acid changes in Addgene's quality control sequence for H2B. It does not alter the function of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH2B-PSmOrange2 was a gift from Vladislav Verkhusha (Addgene plasmid # 34960 ; http://n2t.net/addgene:34960 ; RRID:Addgene_34960)
  • For your References section:

    A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. J Am Chem Soc. 2012 Aug 17. 10.1021/ja3034137 PubMed 22900938