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pPSmOrange2-Tubulin
(Plasmid #34963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34963 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 34963-D50 50 μg of DNA in Tris buffer $495
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DNA 34963-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5328
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSmOrange2
  • Mutation
    R17H, R36H, F64I, Q194L, A224S, G226A (mutations are relative to PSmOrange but numbering is relative to EGFP)
  • GenBank ID
    JQ392581 NM_001018942
  • Tag / Fusion Protein
    • Tubulin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer 5'-cggtaggcgtgtacggtgggag
  • 3′ sequencing primer 5'-gttcagggggaggtgtgggagg
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In Addgene's CMV-F sequencing result, there is a 6 nucleotide deletion corresponding to position 595 of the full plasmid sequence. It is before the ORF and does not appear alter any restriction sites or the function of the plasmid.
https://www.ncbi.nlm.nih.gov/nuccore/JQ392581

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPSmOrange2-Tubulin was a gift from Vladislav Verkhusha (Addgene plasmid # 34963 ; http://n2t.net/addgene:34963 ; RRID:Addgene_34963)

  • For your References section:

    A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. J Am Chem Soc. 2012 Aug 17. 10.1021/ja3034137 PubMed 22900938
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