p6654 MSCV-P C-Flag HA 98E7-Kozak
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35148||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV-P C-Flag-HA
- Backbone size w/o insert (bp) 8700
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameHPV98 E7
SpeciesHuman papillomavirus type 98
Insert Size (bp)300
/ Fusion Proteins
- FLAG (C terminal on backbone)
- HA (C terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer 5' CTACATCGTGACCTGGGAAGC 3'
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p6654 MSCV-P C-Flag HA 98E7-Kozak was a gift from Peter Howley (Addgene plasmid # 35148 ; http://n2t.net/addgene:35148 ; RRID:Addgene_35148)
For your References section:Systematic identification of interactions between host cell proteins and E7 oncoproteins from diverse human papillomaviruses. White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, Harper JW, Howley PM. Proc Natl Acad Sci U S A. 2012 Jan 9. 10.1073/pnas.1116776109 PubMed 22232672