pDZ251 (pKAN 24xPP7SL cyc)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35192||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Miklos & Greg Freyer (Cold Spring Harbor Laboratory)
- Backbone size w/o insert (bp) 4194
Growth in Bacteria
Gene/Insert name24xPP7SL cyc
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13F20 (Common Sequencing Primers)
This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.
The 24xPP7 stem loop cassettes in this plasmid were generated from two non-identical stem-loops to reduce unnecessary redundancy and the chance of recombination in bacteria. The sequence for a single cassette (with the two non-identical stem-loops in uppercase) is:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDZ251 (pKAN 24xPP7SL cyc) was a gift from Robert Singer (Addgene plasmid # 35192 ; http://n2t.net/addgene:35192 ; RRID:Addgene_35192)
For your References section:Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033