Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
(Plasmid #35193)


Item Catalog # Description Quantity Price (USD)
Plasmid 35193 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    David Miklos & Greg Freyer (Cold Spring Harbor Laboratory)
  • Backbone size w/o insert (bp) 4191

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    tdtomato cyc loxP KAN loxP
  • Species

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13rev
  • 3′ sequencing primer M13F20
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid was designed to construct C-terminal fusions of a protein of interest with tdtomato

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ264 (pKAN tdtomato cyc loxP KAN loxP) was a gift from Robert Singer (Addgene plasmid # 35193 ; ; RRID:Addgene_35193)
  • For your References section:

    Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033