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pTH-MYCN
(Plasmid #35416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35416 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 35416-D50 50 μg of DNA in Tris buffer $495
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DNA 35416-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGEM7Zf(+)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2997
  • Modifications to backbone
    TH promoter and Beta-Globin Intron inserted upstream of MYCN cDNA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
  • Alt name
    MYCN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • GenBank ID
    NM_005378
  • Entrez Gene
    MYCN (a.k.a. FGLDS1, MODED, MPAPA, MYCNsORF, MYCNsPEP, N-myc, NMYC, ODED, bHLHe37)
  • Promoter Tryosine Hydroxlyase (TH)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH-MYCN was a gift from William A. Weiss (Addgene plasmid # 35416 ; http://n2t.net/addgene:35416 ; RRID:Addgene_35416)

  • For your References section:

    Targeted expression of MYCN causes neuroblastoma in transgenic mice. Weiss WA, Aldape K, Mohapatra G, Feuerstein BG, Bishop JM. EMBO J. 1997 Jun 2;16(11):2985-95. 10.1093/emboj/16.11.2985 PubMed 9214616
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