Reporter
(Plasmid #36008)

• Depositing Lab: Larry Gerace
• Publication: Ohba et al J Cell Biol. 2004 Dec 20;167(6):1051-62.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression ; Reporter
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAP2β-TM
• Mutation: FRB is residues 2025-2114 of FRAP, LAP2β-TM is residues 401-452 of LAP2β
• Promoter: CMV
• Tags / Fusion Proteins:
  • 11kD FRB (N terminal on insert)
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To be used in conjunction with protein trap construct (Addgene plasmid #36009).