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pEBB-TB
(Plasmid #36099)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36099 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEBB
  • Backbone size (bp) 5450
  • Modifications to backbone
    BT-tag (biotinilation site from P.freudenreichia transcarboxilase, separated from MCS by TEV-site)
  • Vector type
    Mammalian Expression
  • Promoter EF1a
  • Tag / Fusion Protein
    • BT-tag (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CTCAAGCCTCAGACAGTGG
  • 3′ sequencing primer GAGGGGTGCAAGGACCCAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

After mammal transfection, supplement fresh media with 4 uM Biotin to "charge" tag by Biotin. Allow cells to grow for about 48 h before the purification steps.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB-TB was a gift from Ezra Burstein (Addgene plasmid # 36099 ; http://n2t.net/addgene:36099 ; RRID:Addgene_36099)
  • For your References section:

    A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E. Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. 10.1038/nprot.2010.109 PubMed 20671728