|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36187||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7498
Modifications to backboneGolden Gate compatible (see Cermak, et al., 2011) expression vector for TALENs. TAL-DNA binding domain can be removed via BamHI and XbaI digestion.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHomodimeric FokI Nuclease
Mutationdelta152,+63 truncation to TAL backbone
- Promoter TEV
/ Fusion Protein
- ACV5 (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer ttggcgtcggcaaacagtgg
- 3′ sequencing primer ttaaaagtttatctcaccg (Common Sequencing Primers)
Note that there are some minor discrepancies between the Addgene quality control sequence and the sequence from the depositor. These differences will not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZHY501 was a gift from Daniel Voytas (Addgene plasmid # 36187 ; http://n2t.net/addgene:36187 ; RRID:Addgene_36187)
For your References section:TALENs enable efficient plant genome engineering. Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF. Plant Physiol. 2012 Nov 2. 10.1104/pp.112.205179 PubMed 23124327