Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #36195)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 36195 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7325
  • Total vector size (bp) 8036
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    GRLF1 (Codon Devices Synthesized)
  • Alt name
    PDB: 3C5H
  • Alt name
    glucocorticoid receptor DNA binding factor 1 isoform b
  • Alt name
    GRF-1, KIAA1722, MGC10745, P190-A, P190A, p190RhoGAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Contains the Ras homolog domain only (amino acid residues 13-249)
  • Entrez Gene
    ARHGAP35 (a.k.a. GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP, p190RhoGAP)
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GRLF1 was a gift from Cheryl Arrowsmith (Addgene plasmid # 36195 ; ; RRID:Addgene_36195)