pCY 3110-01
(Plasmid
#36236)
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Depositing Lab
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Depositing OrganizationUniversity of Delaware
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 36236-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 36236-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepBS7
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Backbone manufacturerYeast Resource Center, University of Washington
- Backbone size w/o insert (bp) 4882
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Vector typeYeast cassette for PCR and homologous recombination
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Selectable markersGeneticin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha used for DNA recovery of plasmid (i.e. DNA preps).
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemEos2
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Insert Size (bp)708
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Tag
/ Fusion Protein
- yEpolylinker-mEos2-kanMX (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BssHII (unknown if destroyed)
- 5′ sequencing primer CCTTATGTATCATACACATACG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid 20341 (McKinney, S. A., Murphy, C. S., Hazelwood, K. L., Davidson, M. W. and Looger, L. L. (2009). A bright and photostable photoconvertible fluorescent protein. Nat Methods 6, 131-3.)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 36236-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 36236-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCY 3110-01 was a gift from Anne Robinson (Addgene plasmid # 36236 ; http://n2t.net/addgene:36236 ; RRID:Addgene_36236) -
For your References section:
Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS. Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr 4. 10.1002/yea.2895 PubMed 22473760
