Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36321||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7200
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameER-ZapCY1 high affinity Zn(II) sensor
Insert Size (bp)1697
- Promoter CMV
/ Fusion Protein
- KDEL (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Targeted to ER: contains C-terminal KDEL
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-ER-ZapCY1 was a gift from Amy Palmer (Addgene plasmid # 36321 ; http://n2t.net/addgene:36321 ; RRID:Addgene_36321)
For your References section:Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Epub 2011 Apr 18. 10.1073/pnas.1015686108 PubMed 21502528
Map uploaded by the depositor.