pc3xTC20-alphaTubulin
(Plasmid #36327)

• Depositing Lab: Amy Palmer
• Publication: Van Engelenburg et al Chembiochem. 2010 Mar 1;11(4):489-93.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha tubulin 1b with optimized tetracysteine peptides for ReAsH labeling
• Alt name: 3xTC20
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1484
• Entrez Gene: TUBA1B (a.k.a. K-ALPHA-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xTC20 (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry