pMito-LSSmOrange
(Plasmid #37135)

• Depositing Lab: Vladislav Verkhusha
• Publication: Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4039
• Total vector size (bp): 4749
• Modifications to backbone: human cytochrome C oxidase subunit VIII insert
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LSSmOrange
• Insert Size (bp): 710
• Mutation: A44V/F84L/W145M/I165D/M167L/G202D compared to mOrange (but numbering relative to EGFP)
• Promoter: CMV
• Tag / Fusion Protein:
  • human cytochrome C oxidase subunit VIII (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry