Skip to main content
Addgene
Open Menu

YFP-FKBP-C2(Lact)
(Plasmid #37300)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 37300 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    modified pEGFP-C1
  • Modifications to backbone
    EGFP has been replaced with YFP-FKBP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lactadherin C2
  • Mutation
    ~aa 230-387
  • Promoter CMV
  • Tag / Fusion Protein
    • YFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F, EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    YFP-C2(lact) kindly provided by W.D Heo (KAIST, Korea)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR product encoding the C2 domain of lactadherin was inserted into the MCS of a modified pEGFP-C1 vector

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-FKBP-C2(Lact) was a gift from Takanari Inoue (Addgene plasmid # 37300)

  • For your References section:

    Triggering actin comets versus membrane ruffles: distinctive effects of phosphoinositides on actin reorganization. Ueno T, Falkenburger BH, Pohlmeyer C, Inoue T. Sci Signal. 2011 Dec 13;4(203):ra87. 10.1126/scisignal.2002033 PubMed 22169478
Related items: