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pcDNA-D1cpV
(Plasmid #37479)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37479 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3'
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 9500
  • Modifications to backbone
    Note that the N-terminus of pcDNA3’ has the His tag of pRSETB inserted after the HindIII site (There is also a NotI site): AAGCTTGCGGCCGCCAACATGGTGCGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCC
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D1cpV second generation cameleon (calcium sensor)
  • Alt name
    D family cameleon
  • Species
    Synthetic
  • Insert Size (bp)
    1950
  • GenBank ID
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Amy Palmer in the lab of Roger Tsien.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus (at aa 173). D1 does not bind wild-type calmodulin.

See also Palmer & Tsien (2006) Nature Protocols.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-D1cpV was a gift from Amy Palmer & Roger Tsien (Addgene plasmid # 37479 ; http://n2t.net/addgene:37479 ; RRID:Addgene_37479)
  • For your References section:

    Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor. Palmer AE, Jin C, Reed JC, Tsien RY. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Epub 2004 Dec 7. 10.1073/pnas.0408030101 PubMed 15585581